| 11 Sep 2021 | Musselburgh A (ER1) (H) | L 15-24 |
| 18 Sep 2021 | Hawick Force (ER1) (A) |
| 25 Sep 2021 | Currie A (ER1) (H) |
| 2 Oct 2021 | Biggar A (ER1) (A) |
| 9 Oct 2021 | Heriot's A (ER1) (H) |
| 16 Oct 2021 | Edinburgh Accies A (ER1) (H) |
| 23 Oct 2021 | Stewart's Melville A (ER1) (A) |
| 30 Oct 2021 | Boroughmuir A (ER1) (H) |
| 6 Nov 2021 | Jedforest A (ER1) (H) |
| 27 Nov 2021 | Musselburgh A (ER1) (A) |
| 4 Dec 2021 | Hawick Force (ER1) (H) |
| 11 Dec 2021 | Currie A (ER1) (A) |
| 15 Jan 2022 | Biggar A (ER1) (H) |
| 22 Jan 2022 | Heriot's A (ER1) (A) |
| 29 Jan 2022 | Edinburgh Accies A (ER1) (A) |
| 19 Feb 2022 | Stewart's Melville (ER1) (H) |
| 5 Mar 2022 | Boroughmuir A (ER1) (A) |
| 26 Mar 2022 | Jedforest A (ER1) (A) |